Nuclear DNA was prepared from synchronously replicating nuclei of Physarum polycephalum, mixed with cytochrome c and formamide, and spread on the surface of a hypophase consisting of 10 mM Tris, 1 mM ...

Mouse myoblasts stably transfected with GFP-PCNA to label replication foci and the 3D distribution of the GFP signal recorded over time using a confocal microscope. The time series illustrates the dyn...

Mouse myoblast cells were engineered to express GFP-PCNA (proliferating cell nuclear antigen) as a marker for replication factories, and also immunostained for DNA ligase. DNA was localized using Hoec...

Shown are 0.5 micrometer thick confocal image of midplane sections of synchronized 3T3 fibroblasts pulsed for 2 min with CldU and labeled with FITC (green) followed by a variable chase time, and a sec...

HeLa cells expressing a kinetochore-specific protein were synchronized and released into S phase. At time intervals of 1,3,4,6,and 8 hr, cells were pulsed with BrdU, fixed and processed to label contr...

Shown is a 0.5 micrometer thick confocal image of a midplane section of a 3T3 fibroblast pulsed for 5 min with BrdU and immuno-labeled with anti-BrdU antibodies. Replication is confined to discrete si...

Mouse myoblast cells were transfected with GFP-PCNA (proliferating cell nuclear antigen) as a marker for replication factories, pulsed with BrdU, fixed, and immunostained with anti-BrdU. The image sh...

Shown is a chromosome spread of a PtK1 cell pulsed with BrdU in early S phase, then treated with colcemid to arrest cells at metaphase. Spreads were immuno-labeled with FITC-conjugated anti-BrdU antib...

Model of the yeast Origin Recognition Complex ORC)complexed with double stranded DNA incorporating a sequence that encodes the replication origin. The 3D structure of the complex with bound DNA was de...

Figures 228 (upper) and 229 (lower) from Chapter 7 (Mitochondria) of 'The Cell, 2nd Ed.' by Don W. Fawcett M.D. A mitochondrion from rat adrenal tissue grown in vitro shows an unusually high number of...